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RBD-cVLP vaccination reduces lung inflammatory cytokine responses to a lethal challenge with SARS-CoV-2. TNFα, IL-6, IL-1β, <t>IFN-ϒ,</t> <t>and</t> <t>IFN-λ</t> protein levels were quantified by ELISA using the same lung homogenates used for measuring viral titers (see ) extracted from RBD-cVLP-vaccinated and unvaccinated K18-hACE2 mice at days 2 and 4 post challenge with SARS-CoV-2. Data are represented as means (pg/g lung) ± SEM. ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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RBD-cVLP vaccination reduces lung inflammatory cytokine responses to a lethal challenge with SARS-CoV-2. TNFα, IL-6, IL-1β, IFN-ϒ, and IFN-λ protein levels were quantified by ELISA using the same lung homogenates used for measuring viral titers (see ) extracted from RBD-cVLP-vaccinated and unvaccinated K18-hACE2 mice at days 2 and 4 post challenge with SARS-CoV-2. Data are represented as means (pg/g lung) ± SEM. ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Vaccines

Article Title: Protection of K18-hACE2 Mice against SARS-CoV-2 Challenge by a Capsid Virus-like Particle-Based Vaccine

doi: 10.3390/vaccines12070766

Figure Lengend Snippet: RBD-cVLP vaccination reduces lung inflammatory cytokine responses to a lethal challenge with SARS-CoV-2. TNFα, IL-6, IL-1β, IFN-ϒ, and IFN-λ protein levels were quantified by ELISA using the same lung homogenates used for measuring viral titers (see ) extracted from RBD-cVLP-vaccinated and unvaccinated K18-hACE2 mice at days 2 and 4 post challenge with SARS-CoV-2. Data are represented as means (pg/g lung) ± SEM. ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: ELISA kits specific for mouse IFN-β (DY8234-05, R&D Systems, Minneapolis, MN, USA), TNF-α (DY410-05, R&D Systems, Minneapolis, USA), IL-6 (DY406-05, R&D Systems, Minneapolis, MN, USA), IFN-λ (D485-05, R&D Systems, Minneapolis, MN, USA), IFN- Υ (DY1789B-05, R&D Systems, Minneapolis, USA), and IL-1β (D401-05, R&D Systems, Minneapolis, USA) were used following the manufacturer’s specifications.

Techniques: Enzyme-linked Immunosorbent Assay